PlateFlo - Plate Scale Perfusion System

A modular system for programmable, plate-scale perfusion of adherent cell lines.

File Repository: OSF (Open Science Foundation)

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This documentation is written to accompany PlateFlo – A software-controllable plate-scale perfusion system for culture of adherent cells, published in HardwareX (Oct 2021). See the peer-reviewed article for a detailed description and contextualization of the hardware.

Left Schematic overview of a multi-plate multi-reservoir perfusion setup controlled programmatically using the PlateFlo python package. Right PlateFlo perfusion plate. A modified Nunc® OmniTray® culture plate.

Hardware

• Bill of Materials (BOM)
  • BOM Tables
  • Required Tools
  • Optional Tools/Components
• Design Files
• Build Guide
  • 1. 3D Printing Guidelines
  • 1. FETbox Hardware Controller
  • 3. Perfusion Plate
• Operating Instructions
  • 1. System Setup
  • 2. Inlet/Outlet Flow Rate Delta
  • 3. Skimmer Pump Operation
  • 4. Example Perfusion Setups
  • 5. Appendix

PlateFlo Python Package

• FETbox Serial Control
  • 1. Key Features
  • 2. Quick Start
  • 3. Usage
  • 4. Expanding Functionality
• Perfusion Event Scheduler
  • 1. Key Features
  • 2. Quick Start
  • 3. Usage
  • 4. Working Examples
• PlateFlo API Index
  • fetbox
  • scheduler
  • ismatec
  • serial_io

Legal

• License
  • Hardware
  • Software
• Disclaimer

Indices and Tables

• Index

• Module Index

• Search Page